The Complete Peptide Dosing Guide: From Beginner to Advanced Protocols

The Foundation: Understanding Units and Measurements

Before we pick up a syringe, we must define our terms. In the world of peptides, we deal primarily with two measurements: Milligrams (mg) and Micrograms (mcg).

Think of a Milligram as a shipping container. Inside that container are 1,000 individual boxes called Micrograms.

• 1 mg = 1,000 mcg
• 5 mg = 5,000 mcg

Most vials you purchase, like a 50mg vial of Epitalon, are labeled in milligrams. However, your daily dose is almost always measured in micrograms. If you confuse these two, you are essentially trying to fit a shipping container into your garage: it’s a recipe for disaster.

The Equipment: The U-100 Syringe

Most research is conducted using U-100 Insulin Syringes. These are standard 1mL syringes divided into 100 “units.”

Focus on this: A “unit” is not a fixed amount of a drug; it is a measurement of volume. How much peptide is in one unit depends entirely on how much liquid (Bacteriostatic Water) you added to the vial.

Problem-Solution: The Reconstitution Math

The Problem: You have a 5mg vial of dry powder. You need a 250mcg dose. How many units do you pull into the syringe?

The Solution: Follow the three-step “Concentration Logic.”

Step 1: Convert the Vial to Mcg

Take the mg on the label and multiply by 1,000.

• 5mg x 1,000 = 5,000 mcg total.

Step 2: Determine Your Concentration

Decide how much Bacteriostatic (BAC) water to add. Adding 2mL (200 units) is a standard industry practice because it makes the math easy to digest.

• 5,000 mcg / 2mL = 2,500 mcg per mL.

Step 3: Find Your Unit Value

Since a U-100 syringe has 100 units in 1mL, we divide our concentration by 100.

• 2,500 mcg / 100 units = 25 mcg per unit.

The Worked Example:
If 1 unit = 25 mcg, and your target dose is 250 mcg, you simply divide:

• 250 / 25 = 10 units.